This web page was produced as an assignment for Gen677 at UW-Madison Spring 2009.
Profile Structure Homology Phylogeny
Alignments
Since a DNA sequence can be translated in different frames and spliced in different ways, it would be interesting to see how similar the protein sequences of SRD5A2 would across the model organisms previously analyzed as compared to the genetic similarities.
ClustalW
| clustalw.doc | |
| File Size: | 29 kb |
| File Type: | doc |
T-COFFEE
| t-coffee.pdf | |
| File Size: | 29 kb |
| File Type: | |
Muscle
| muscle.doc | |
| File Size: | 37 kb |
| File Type: | doc |
As far as the individual programs go, T-COFFEE again was my favorite. The results are in .pdf and the color coding makes them very easy to read. As far as the sequence, they seem to be much more similar than when using the DNA sequence. Since each of the enzymes functions in such a similar way, it makes sense that they have very conserved amino acids sequences. Regardless, to a certain extent of course, of the genetic sequence the translated sequences are very similar.
References
1. Dereeper A.*, Guignon V.*, Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.F., Guindon S., Lefort V., Lescot M., Claverie J.M., Gascuel O. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9. Epub 2008 Apr 19. (PubMed) *: joint first authors
